The Enzymatic Conversion of N5-Formyl Tetrahydrofolic Acid (Folinic Acid) to N10-Formyl Tetrahydrofolic Acid
نویسندگان
چکیده
منابع مشابه
Enzymatic reduction of folic acid and dihydrofolic acid to tetrahydrofolic acid.
Earlier reports on the inactivation of FA’ in the presence of liver extracts indicated the supplements required (1, 2) and described the enzymatic conversion of FA to a labile compound possessing the properties of FAH4 (3). The reduction of FA by TPNH has been noted by Miller and Waelsch (4). Recently, the enzymatic reduction of FA to FAHz by a bacterial extract was described by Wright and Ande...
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The enzymatic formation of PO-CHOeFA’ from FA with FAG as the specific formyl donor and with a soluble liver extract as the source of enzymes has been described in the preceding paper (1). The specific l-carbon acceptor appeared to be FAHd which functioned catalytically. When FAG and substrate amounts of FAHd were incubated with enzyme and the reaction mixture acidified, ACF could be detected a...
متن کاملThe dissociation constants of tetrahydrofolic acid.
Six dissociable groups exist in tetrahydrofolic acid in the range Ho -3.0 to pH 14. The assignments in tetrahydrofolic acid by titrimetric and spectrophotometric comparisons with model compounds, +uninobenzoylglutamic acid and 2-amino-6,7-dimethyl-5,6,7,8-tetrahydro-4-pteridinone, are as follows: acidic ionization of amide, pK’ 10.5; Ns, pK’ 4.82; y-carboxyl, pK’ 4.8; cr-carboxyl, pK’ 3.5; N1, ...
متن کامل5-Methyl-5,6,7,8-tetrahydrofolic acid. Conformation of the tetrahydropyrazine ring.
It is suggested from analysis of proton spin-spin coupling constants that the tetrahydropyrazine ring of 5methyltetrahydrofolate in solution is in a half-chair conformation, with the methyl group at N-5 and the methylene group C-9 truns, and with C-6-H in an equatorial position. The chemical shifts and spin-spin coupling constants for the carbon-bound hydrogens of (+)-L-, (-)-L, (+)-L-~-‘H, 7-‘...
متن کاملThe mechanism of the condensation of formaldehyde with tetrahydrofolic acid.
The bell-shaped pH rate profile for the reaction of tetrahydrofolic acid with formaldehyde to form 5, lo-methylene tetrahydrofolic acid has been shown to result from a change in rate-determining step with changing acidity. In alkaline solution, the rate-determining step is the acid-catalyzed dehydration of hydroxymethyl tetrahydrofolic acid and its conjugate base. This step is subject to genera...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1960
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)69609-7